The in vivo optical imaging facility includes a state-of-the-art IVIS SPECTRUM (Perkin Elmer) whole-mouse imaging system with 3D, fluorescence, bioluminescence, and Cerenkov luminescence capabilities for high throughput imaging, in vitro (microplates) and in vivo in live animals (5 mice, 23cm FoV). For advanced fluorescence imaging, the IVIS Spectrum has capabilities for either trans-illumination (from the bottom) or epi-illumination (from the top) of in vivo fluorescent sources. 3D diffuse fluorescence tomography can be performed to determine source localization and concentration using a combination of structured light and trans-illumination fluorescent images.